Brigham & Women's Hospital Clinical Pathology & Laboratories

Additional (Reflex) Testing Policy

To diagnose certain disease states additional testing is necessary; therefore, to avoid delay in the turnaround time of such testing, and the necessity of collecting new specimens,  certain protocols and procedures will be followed.
 
If the test
has a result of
perform additional test:
Serum Protein Electrophoresis (SPEP) monoclonal spike Immunofixation (IFX) to look for IgG, IgA and IgM monoclonal gammopathies
SPEP (Hematology patients only) monoclonal spike Immunofixation for IgG, IgA and IgM
ANA positive Precipitin Panel, anti-dsDNA
Urine Protein Electrophoresis (UPEP) monoclonal spike Immune electrophoresis
CH50 less than 150 u/mL C1Q, C2, C3, C4, Factor B for BWH and NE Deaconess patients
Lyme Antibodies (ELISA screen) positive Confirmation by western blot
CBC with automated differential 2+ left shift, IG or positive flag for blasts Manual Differential
Urinalysis positive for protein, blood or leukocyte esterase urine sediment analysis (microscopic urine exam)
Paroxysmal Nocturnal Hemoglobinuria (PNH; Sucrose Lysis) positive acid serum
Osmotic Fragility and Coombs Test positive and negative, respectively autohemolysis
PTT/Lupus anticoagulant positive STATCLOT/Lupus anticoagulant confirmatory
von Willebrand Panel and platelet aggregation studies ordered together Ristocetin titration to complete information needed for accurate diagnosis
HIV(EIA) positive confirmation by western blot
Fetal Lung Maturity (FLM) All diabetic patients and/or results of 40-59 mg/G DSPC assay
Activated protein C resistance (screen) positive PCR for Factor V Leiden mutation/Activated Protein C Resistance
Anti-thrombin III functional assay abnormal Antigenic assay for AT-III
Protein C, functional assay abnormal Antigenic assay for protein C
Hemoglobin Electrophoresis A2 > 3.5% A2 Column
Antibody Screen Positive Antibody Identification (Pregnant women with a clinically significant antibody will have an antibody titer sent to the Center for Blood Research (CBR)
Direct Antiglobulin Test Positive Monospecific Direct Antiglobulin tests with Anti-IgG and Anit-Complement
Monospecific IgG Direct Antiglobulin Test Positive Eluate
RPR Positive TP-PA confirmation sent to the State Laboratory
Bacterial Culture Significant growth Antibiotic susceptibility test(s)
Bacterial Culture Beta hemolytic streptococci Strep Grouping
Bacterial Culture Significant growth unidentified by routine methods Extended aerobic/anaerobic workup
GC Culture Suspected gonococci GC confirmation
Mycobacterial Culture Acid fast growth Genetic probe for AFB
Fungal Culture Yeast (not Candida albicans) Fungus identification
Fungal Culture Fungi (not yeast) Extended mycology workup
Anaerobic Culture Significant anaerobic growth Anaerobic organism ID
Viral Isolations Positive CPE Confirmation by monoclonal antibody staining